Leptospira spp.: Methods and Protocols (Methods in Molecular Biology, 2134)
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Leptospira spp.: Methods and Protocols (Methods in Molecular Biolog...
Book Details
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Publisher: Humana
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Author: Nobuo Koizumi
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Language: English
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Edition: 1st ed. 2020
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ISBN: 9781071604588
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Pages: 301
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Cover: Hardcover
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Dimensions: 10.3 x 7.2 x 0.9 inches
About The Book
Leptospira spp.: Methods and Protocols, edited by Nobuo Koizumi, presents a detailed and practical guide to the isolation, cultivation, and advanced study of Leptospira species, which are responsible for the globally significant zoonotic disease, leptospirosis. This volume is part of the renowned Methods in Molecular Biology series and is crafted for researchers engaged in both foundational and applied microbiology.
The book features thoroughly explained protocols, ranging from whole genome sequencing and MALDI-TOF mass spectrometry for bacterial identification, to gene inactivation techniques and in vitro/in vivo pathogenesis assays. It includes specialized procedures for using hamsters in virulence and vaccine efficacy testing, making it an indispensable reference for vaccine development and pathogen study.
Each chapter is designed with reproducibility in mind, offering detailed steps, necessary materials, and expert troubleshooting tips. Whether for researchers new to the field or experienced microbiologists seeking refined methods, this book is a cutting-edge resource for anyone exploring the complexities of Leptospira biology and pathogenesis.
